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    2020-9-18 · Plate 100 μL of this mixture onto LB + kanamycin plates to select for kanamycin resistant phage lysogens. 54. Incubate plates at 37°C for 16 h. Note: If working with a temperature sensitive mutant of cI such as cI857, then this step should be performed at 30°C. 55. Pick several colonies with an inoculating loop and resuspend in 20 μL of H 2 ...

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    2020-6-18 · Briefly, before colonization, 8-10-week-old C57BL/6 mice were fasted for 4 hrs followed by gavage of kanamycin (20 mg). After 20 hrs, mice were fasted again for 4 hrs and administrated with 1×10 4 kanamycin resistant E. coli (BW25113 wild-type carrying pET-28a (+) plasmid). Fecal pellets were collected 7 days post colonization and sonicated in ...

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    2020-2-15 · Kanamycin Injection, USP, sterile solution for parenteral administration, contains respectively; kanamycin sulfate equivalent to 500 mg and 1g kanamycin; sodium bisulfite, an antioxidant, 0.66% and 0.45%; and sodium citrate, 2.2% and 2.2% with …

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    kanamycin has been employed to treat human tuberculosis in combination with other effective drugs. because the therapy of this disease is protracted & involves admin of large total doses of drug, with risk of ototoxicity & nephrotoxicity, /it should be used only to treat patients who harbor microorganisms that are resistant to the more commonly used agents.

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    INSTRUCTIONS FOR USE – READY-TO-USE BOTTLED MEDIA BA-257107.06 Rev.: March 2019 BA-257107.06 Page 1 of 3 BD Tryptic Soy Broth (TSB) INTENDED USE BD Tryptic Soy Broth (Soybean-Casein Digest Medium) is a general purpose liquid enrichment medium used in qualitative procedures for the sterility test and for the enrichment and cultivation

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    2003-8-26 · Plates are immediately sealed in plastic bags and frozen at or below −20 °C. A number of commercial manufacturers offer microdilution plates with agents dried into the wells which, after reconstitution with broth (usually 50 to 200 µL), provide selected concentrations of agents. These plates must be stored as recommended by the manufacturer.

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    2015-12-21 · The API monographs state that kanamycin monosulfate contains not less than 750 IU per mg (with reference to the dried substance) and kanamycin acid sulfate not less than 670 IU per mg (with reference to the dried substance). Members of the Expert Committee received the text of the current monograph with proposed corrections indicated in the text.

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    2021-6-30 · The MAUDE database houses medical device reports submitted to the FDA by mandatory reporters 1 (manufacturers, importers and device user facilities) and voluntary reporters such as health care professionals, patients and consumers.

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    2016-9-30 · CAS:491-80-5. Molecular Formula: C16H12O5. Molecular Weight: 284.26. Melting Point:210-213 °C (lit.) Boiling Point:518.6 °C at 760 mmHg. Flash Point:198.2 °C. Product description: Have estrogen-like effect, can arrest the access of cholesterol, aswell has antifungal and antitumor action. The hormone levels in the animal physique has a two ...

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    2020-8-25 · Reconstitute polymyxin B powder for injection by dissolving 500,000 units of the drug in 300–500 mL of 5% dextrose injection to provide solutions containing approximately 1000–1667 units/mL. Rate of Administration. IV infusions of polymyxin B usually are given over 60–90 minutes. An infusion period <30 minutes should not be used. Because ...

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