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cost of kanamycin monosulfate tokyo

  • Kanamycin Monosulfate 25389-94-0 | Tokyo Chemical

    Kanamycin, an aminoglycoside antibiotic, was first isolated in 1957 from the bacterium Streptomyces kanamyceticus by Umezawa. Kanamycin has broad activity against Gram-negative bacteria and Gram-positive bacteria, including Mycobacterium tuberculosis. Kanamycin interacts with the 30S subunit of prokaryotic ribosomes, and then it inhibits bacterial growth by inhibiting protein synthesis.

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  • Kanamycin Monosulfate 25389-94-0 | Tokyo Chemical ...

    2941.90-000. *이 HS 번호 는 TCI (일본)에서 제품을 수출할 때만 사용됩니다. 이용 예. Application. Kanamycin: A Broad Spectrum Aminoglycoside Antibiotic and a Selective Reagent Genetically Engineered Cells. Kanamycin, an aminoglycoside antibiotic, was first isolated in 1957 from the bacterium Streptomyces kanamyceticus by Umezawa.

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  • Kanamycin Monosulfate 50 mg/mL Solution | GoldBio

    Consequently, protein synthesis is inhibited. GoldBio’s antibiotic solutions are provided ready to use. Product Specifications: Kanamycin Monosulfate in H 2 O. (50 mg/mL Premade Solution, 0.2 µm Sterile Filtered) MOLECULAR BIOLOGY GRADE. Formula: C 18 H 36 N 4 O 11 · H 2 SO 4. MW: 582.58 g/mol.

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  • Kanamycin Monosulfate - OEL Fastrac with ADE -

    2021-6-15 · OEL Fastrac Catalog. 639. ADD TO CART. Catalog Search. Please note, this OEL/ADE monograph also applies to kanamycin base (CAS RN 59-01-8). Kanamycin sulfate is an aminoglycoside antibiotic produced by Streptomyces kanamyceticus indicated for susceptible infections.

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  • Kanamycin A Monosulfate - LKT Labs

    2021-5-30 · Kanamycin A is an aminoglycoside antibiotic that binds the bacterial 16s rRNA subunit, inhibiting translocation and degrading target RNA; it displays antibacterial efficacy against gram negative bacteria. Kanamycin also prevents formation of the initiation complex. Kanamycin A also inhibits mammalian RNA splicing.

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  • Kanamycin sulfate | ≥99%(HPLC) | Selleck | Selection ...

    Kanamycin Monosulfate is an aminoglycoside bacteriocidal antibiotic. It is used to treat several infections and as a selective agent in molecular biology to isolate bacteria. Ungraded products supplied by TCI America are generally suit. Pricing & More Info bioWORLD Murashige AND Skoog (MS) Medium Plates, w Kanamycin ...

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  • Kanamycin | Spectrum

    Buy highly pure kanamycin monosulfate (861RB), from Pharmaffiliates. Login as registered user for prices, availability and discounts.

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  • Product Name : kanamycin monosulfate (861RB) |

    THE CRYSTAL STRUCTURE OF KANAMYCIN Gunji Koyama and Yoichi litaka Faculty of Pharmaceutical Sciences, University of Tokyo Bunkyou, Tokyo, Japan Kenji Maeda and Hamao Umezawa Institute of Microbial Chemistry Shinagawa-Ku, Tokyo, Japan (Received in Japan 5 December 1967) The structure (1) of kanamycin has been proposed by the chemical studies (1,2,3)In the present paper, the authors report the crystal structure determination of kanamycin …

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  • The crystal structure of kanamycin - ScienceDirect

    2006-12-11 · Kanamycin, an antibiotic complex produced by Streptomyces kanamycetius isolated from Japanese soil, was described by Okami and Umezawa as early as 1957 and consists of three components: Kanamycin A (the major component), B, and C. The disulfate salt of kanamycin A [4- O - (6-amino-6-deoxy-α- d -glucopyranosyl)-6- O - (3-amino-3-deoxy-α- d ...

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  • Product Name : kanamycin monosulfate (861RB) |

    Buy highly pure kanamycin monosulfate (861RB), from Pharmaffiliates. Login as registered user for prices, availability and discounts.

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  • Kanamycin Monosulfate - OEL Fastrac with ADE -

    2021-6-15 · Kanamycin Monosulfate. OEL Fastrac Catalog. 639. ADD TO CART. Catalog Search. Please note, this OEL/ADE monograph also applies to kanamycin base (CAS RN 59-01-8). Kanamycin sulfate is an aminoglycoside antibiotic produced by Streptomyces kanamyceticus indicated for susceptible infections. Kanamycin, an aminoglycoside, acts by inhibiting the ...

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  • Product Name : Kanamycin B sulfate (K0100000 ...

    Buy highly pure Kanamycin B sulfate (K0100000), from Pharmaffiliates. Login as registered user for prices, availability and discounts.

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  • Kanamycin sulfate | ≥99%(HPLC) | AdooQ®

    2021-6-1 · CAS NO. 25389-94-0. Kanamycin sulfate is an aminoglycoside bacteriocidal antibiotic. Next day delivery by 10:00 a.m. Order now. WARNING: Attention California Residents: This product can expose you to chemicals including Kanamycin sulfate , which is known to the State of California to cause cancer and birth defects or other reproductive harm.

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  • LB Agar Kanamycin-50, Plates pre-poured LB agar

    Suitable for selective cultivation of E. coli strains that contain plasmids conferring kanamycin resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins. LB Agar Kanamycin-50, Plates has been used in the screening of Agrobacterium tumefaciens and E.coli JM83 strain with pGD103 plasmid.

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  • Potentiometric Adsorption Isotherm Analysis of a ...

    2021-3-12 · Paromomycin sulfate, kanamycin monosulfate, 4-vinylphenylboronic acid (VPBA), and N,N,N′,N′-tetramethylethylenediamine (TEMED) were purchased from Tokyo Chemical Industry Co., Ltd. N-3-(Dimethylamino)propylmethacrylamide (DMAPMAAm), dimethyl sulfoxide (DMSO), MAA, N,N′-methylenebisacrylamide (MBAAm), 2-hydroxyethyl methacrylate (HEMA ...

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  • Measurement of the Promoter Activity in Escherichia

    2020-1-20 · 50 mg/ml kanamycin. Dissolve 0.5 g of kanamycin monosulfate (Nacalai Tesque) in 10 ml of distilled water. Sterilize the solution by filtration with 0.22 μm filter (Advantec) Store the sterilized solution at 4 °C. LB broth. Dissolve 10 g of Bacto TM tryptone, 5 g of Bacto TM yeast extract, and 10 g of NaCl in 800 ml of distilled water

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  • Resonance light scattering method for detecting

    In this paper, a resonance light scattering (RLS) method was developed for detecting kanamycin with high sensitivity and selectivity. Here, aptamer specific to kanamycin was utilized for recognizing and competitively binding with kanamycin, and gold nanoparticles (GNPs) were used as probes. After adding kanamycin into solutions containing aptamer and GNPs, the GNPs would aggregate in the ...

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  • Large-Scale Assessment of the Zebrafish Embryo as a ...

    There is an unmet need for low-cost, high-throughput animal models in some fields of biomedical research such as drug screening and toxicity assessment [1,2]. The zebrafish embryo is emerging as one such model [1]. It has been proposed as a bridge ... 23 Kanamycin monosulfate 0 6 13 38 38 060261261156146617965

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  • Genetic tools for advancement of Synechococcus sp.

    2016-11-10 · Successful implementation of modified cyanobacteria as hosts for industrial applications requires the development of a cyanobacterial chassis. The cyanobacterium Synechococcus sp. PCC 7002 embodies key attributes for an industrial host, including a fast growth rate and high salt, light, and temperature tolerances. This study addresses key limitations in the advancement of Synechococcus …

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  • Resonance light scattering method for detecting

    In this paper, a resonance light scattering (RLS) method was developed for detecting kanamycin with high sensitivity and selectivity. Here, aptamer specific to kanamycin was utilized for recognizing and competitively binding with kanamycin, and gold nanoparticles (GNPs) were used as probes. After adding kanamycin into solutions containing aptamer and GNPs, the GNPs would aggregate in the ...

    Get Price
  • Modulation of kanamycin B and kanamycin A

    2017-7-28 · Kanamycin B is a precursor for semisynthetic antibiotics such as arbekacin and dibekacin [3–5] and is generally extracted from the broth of S.kanamyceticus.However, the yield of kanamycin B is too low for industrial applications, necessitating strain improvements to maximize kanamycin B production [6,7].Due to differences in their C2′ substituents, kanamycin B is less biologically active ...

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  • Kanamycin sulphate | VWR

    Kanamycin sulphate 50 mg/ml in solution, Ultra Pure Grade. Catalog Number: (E713-20ML) Clearance. Supplier: VWR Chemicals. Description: Kanamycin is an aminoglycoside antibiotic that inhibits protein synthesis in gram-negative and gram-positive bacteria and in mycoplasma.

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  • Kanamycin sulfate | ≥99%(HPLC) | AdooQ®

    2021-6-1 · CAS NO. 25389-94-0. Kanamycin sulfate is an aminoglycoside bacteriocidal antibiotic. Next day delivery by 10:00 a.m. Order now. WARNING: Attention California Residents: This product can expose you to chemicals including Kanamycin sulfate , which is known to the State of California to cause cancer and birth defects or other reproductive harm.

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  • A novel mouse model of rectal cancer established by ...

    K.K.) and kanamycin monosulfate (KM, 100 µg/ml, Sigma-Al-drich Japan Co., Tokyo) at 37°C in a humidified atmosphere of 5% CO 2 and air. The monolayer culture on plastic dishes (Asahi Techno Glass Co., Tokyo) was washed once with PBS and then harvested after incubation with 1 ml of Cell Dissociation Solu-tion (Sigma-Aldrich Japan Co.).

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  • Effects of Prolonged Kanamycin Administration on

    CAS: 25389-94-0 MDL: MFCD00070253 Synonyms: Kanamycin Monosulphate, Kanamycin A Kanamycin Monosulfate is an aminoglycoside bacteriocidal antibiotic which acts as an inhibitor of protein biosynthesis by producing a misreading of the 70s-ribosome. Kanamycin Monosulfate used in biotechnology applications to inhibit protein synthesis. It is also used as a selection agent for cells …

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  • Kanamycin sulphate ∼735 μg/mg (from Streptomyces ...

    0.93 mg·L–1 (5 μM) NAA, 25 mg·L–1 kanamycin monosulfate, 500 mg·L–1 Claforan (Pharmacia and Upjohn Ltd., Tokyo), and 0.25% (w/v) Gelrite. When adventitious shoots appeared, cultures were transferred to an antibiotic selection medium containing 50 mg·L–1 kanamycin monosulfate and then incubated at 25 ºC

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  • Kanamycin sulphate Cell Culture Reagent | VWR

    CAS: 25389-94-0 MDL: MFCD00070253 EINECS: 246-933-9 Synonyms: Kanamycin Monosulphate, Kanamycin A

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  • Kanamycin sulphate | VWR

    Kanamycin sulphate 50 mg/ml in solution, Ultra Pure Grade. Catalog Number: (E713-20ML) Clearance. Supplier: VWR Chemicals. Description: Kanamycin is an aminoglycoside antibiotic that inhibits protein synthesis in gram-negative and gram-positive bacteria and in mycoplasma.

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  • Aminoglycoside Antibiotics Inhibit Shoot Regeneration

    The cost of publishing this paper was defrayed in part by the payment of page charges. ... Kanamycin at 100 mg·L–1 or higher ... cin monosulfate (100–250), geneticin disul-fate (1–10), neomycin sulfate (200–500), and paromomycin sulfate (100–400). A completely

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  • CAS No.59-01-8,D-Streptamine,O-3-amino-3-deoxy-a

    Kanamycin (CAS NO.59-01-8) is for the production of sulfuric acid amikacin, kanamycin monosulfate, and pairs of kanamycin sulfate intermediates. It is used in molecular biology as a selective agent most commonly to isolate bacteria (e.g., E. coli) which have taken up genes (e.g., of plasmids) coupled to a gene coding for kanamycin resistance ...

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  • Kanamycin B | CAS#4696-76-8 | protein translation ...

    Kanamycin B is an inhibitor of protein translation and mammalian RNA splicing. It inhibits growth of gram negative bacteria, prevents formulation of the initiation complex, and decreases action potential amplitude in cardiac pacemaker cells.

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  • Resonance light scattering method for detecting

    In this paper, a resonance light scattering (RLS) method was developed for detecting kanamycin with high sensitivity and selectivity. Here, aptamer specific to kanamycin was utilized for recognizing and competitively binding with kanamycin, and gold nanoparticles (GNPs) were used as probes. After adding kanamycin into solutions containing aptamer and GNPs, the GNPs would aggregate in the ...

    Get Price
  • Molecules | Free Full-Text | Determination of

    2005-1-1 · The primary goal of this study was the production of liposomes encapsulating kanamycin for drug administration by inhalation. The selected drug is indicated for multiresistant tuberculosis, and administration through inhalation allows both local delivery of the drug to the lungs and systemic therapy. The ethanol injection method used for the liposome production is easily scaled up and is ...

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  • Kanamycin incorporation in lipid vesicles prepared by ...

    5 The InTernaTIonal PharmacoPoeIa The International Pharmacopoeia is a collection of quality specifications for pharmaceutical substances (active ingredients and excipients) and dosage forms, together with supporting general methods of analysis.

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  • Dulbecco′s Phosphate Buffered Saline - DPBS

    单硫酸卡那霉素的杂质谱 1. 上海市食品药品检验所,上海 201203;2. 复旦大学化学系,上海 200433 Impurity Profile of Kanamycin Monosulfate 1. Shanghai Institute for …

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  • verexpression of MdMADS5,an -like gene of apple,

    Dulbecco′s Phosphate Buffered Saline, Modified, with 36 mg sodium pyruvate, 50 mg streptomycin sulfate, 100 mg kanamycin monosulfate, 1000 mg glucose/L and CaCl2, liquid, sterile-filtered, suitable for cell culture. View Pricing.

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  • Development of Structure-Switching Aptamers for

    2002-11-20 · Tokyo, Japan), 0.8% agar (Difco Laboratories, Detroit, MI, USA), 25 mgml 1 kanamycin monosulfate (Meiji Seika Kaisha Ltd, Tokyo, Japan) and 50 mgml 1 claforan (Pharmacia and Upjohn Ltd., Tokyo, Japan). Transformants were identified as kanamycin-resistant seedlings that produced green leaves and well-estab-lished roots in the medium.

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  • Kanamycin Ototoxicity in Healthy Men | JAMA

    Kanamycin was discovered by Umezawa in the course of a systematic screening of organisms that produced antibiotics effective against Gram-positive and Gram-negative bacteria including tubercle bacilli. He had hoped to find one that would not exhibit the delayed toxicity that characterizes other such...

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  • GV3101 Agrobacterium Electrocompetent Cells |

    GV3101 Agrobacterium Electrocompetent Cells have high transformation efficiency such as cDNA and gDNA library construction and are routinely used for Agrobacterium-mediated transformation of Arabidopsis thaliana, tobacco, potato and soybeans.

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  • WHO COLLABORATING CENTRE FOR CHEMICAL

    The total cost for running the Centre in 2004 was estimated to US 649 000. The income from sales of reference substances was US 64 800, the contribution from SIDA was US 274 000 and the contribution received from the WHO headquarters was US 18 100. The deficit of US 292 000 was covered by the support from Apoteket AB.

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  • PHARMACEUTICAL PREPARATIONS - WHO

    5 The InTernaTIonal PharmacoPoeIa The International Pharmacopoeia is a collection of quality specifications for pharmaceutical substances (active ingredients and excipients) and dosage forms, together with supporting general methods of analysis.

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  • Rapid (30-second), equipment-free purification of

    2020-10-1 · In a laminar flow hood, add 100 µl of 50 mg/ml kanamycin monosulfate (in water) to 100 ml of LB medium. The kanamycin-containing LB medium can be stored at 4 °C for up to 2 weeks.

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  • Company Overview - Bevenovo Corporation Limited

    Bevenovo is mainly engaged in produce and sales for feed additive and materials, the core products including fish meal, feather meal, blood meal, meat and bone meal, corn gluten meal and feed yeast, by working with our abroad branch office and distributors, we are now getting more market share and known by more customersWe carry out the market and customer oriented management, based on our own ...

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  • Dulbecco′s Phosphate Buffered Saline - DPBS

    Dulbecco′s Phosphate Buffered Saline, Modified, with 36 mg sodium pyruvate, 50 mg streptomycin sulfate, 100 mg kanamycin monosulfate, 1000 mg glucose/L and CaCl2, liquid, sterile-filtered, suitable for cell culture. View Pricing.

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  • Bacterial Expression of a Single-Chain Variable Fragment ...

    120 rpm) in LB medium (50 µg/mL kanamycin). An aliquot of the culture (400 µL) was transferred to 40 mL of fresh LB medium (50 µg/mL kanamycin). The flask was shaken continu-ously (120 rpm, 37°C) until the optical density (OD 600 ® ® ®

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  • Percellome toxicogenomics project as the 3R-toxicology

    2014-11-20 · Kanamycin monosulfate Levothyroxine Maltol MEHP Menthyl Valerate Methanol Methoprene Methoprene acid Methyl dihydro jasmonate 3-methylcholanthrene Monocrotaline Nerolidol N-ethyl-N-nitrosourea N-Methylaniline Omeprazole Paclitaxel (Taxol) Paraquat dichloride Pentachlorophenol Permethrin Phenobarbital sodium Phenytoin Phytol

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  • (Wr SUBSTANCES - crs.edqm.eu

    Form Crystalline monosulfate-monohydrate Ia1 2% +118' (c. 1.0, water) Sulfate content 16.1% Sulfated ash 0% Kanamycin A content 79.0% In addition to sulfate, the sample contains water of crystallization (theoretical: which could not be detected on 'Loss on drying'.' Y%), The issue af this document doer not constitute formal publication.

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  • Development of Structure-Switching Aptamers for

    2015-1-30 · This work was the first to report that the kanamycin-binding DNA aptamer (5′-TGG GGG TTG AGG CTA AGC CGA-3′) can form stable parallel G-quadruplex …

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  • Label-free detection of kanamycin based on a G

    2021-5-20 · Phages, such as those infecting Bacteroides spp., have been proven to be reliable indicators of human fecal contamination in microbial source tracking (MST) studies, and the efficacy of these MST markers found to vary geographically. This study reports the application and evaluation of candidate MST methods (phages infecting previously isolated B. fragilis strain GB-124, newly isolated ...

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  • Frontiers | Evaluation of Low-Cost Phage-Based

    KANAMYCIN MONOSULFATE (NON-STERILE) 'MEIJI' 許可證字號 衛署藥輸字第003230號 許可 證 有效日期 2003-12-23

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  • Lipopolysaccharide-Deficient Acinetobacter

    2011-9-20 · Gene clusters found in bacterial species classified as Streptomyces encode the majority of known antibiotics as well as many pharmaceutically active compounds. A site-specific recombination system similar to those that mediate plasmid conjugation was engineered to catalyze tandem amplification of one of these gene clusters in a heterologous Streptomyces species.

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  • Two Distinct Signaling Pathways in Hair Cycle Induction ...

    (Kyokuto Pharmaceutical, Tokyo) supplemented with 0.1 mM monoethanolamine/0.1 mM phosphoryl ethanolamine/0.5 ,M hydrocortisone/60 ,ug/ml kanamycin monosulfate at 37?C under an atmosphere of 5% C02/95% air. After being cultured in dishes precoated with collagen type I (Iwaki Glass, Tokyo…

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  • Three dimensional M × N type aptamer-functionalized

    chain reaction (PCR) at low cost; aptamers also possess higher affinity, speci city, and stability with targets.18,19 Aptamers have been employed for speci cally recognizing antibiotics, e.g. chloramphenicol,20,21 oxytetracycline22 and kanamycin,23,24 in food. In addition, Mehta's group reported a DNA aptamer that

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  • A.T.Chem Supplier Asia Talent Chemical

    2014-10-29 · Kanamycin Mono Sulfate: 04SC008: 25389-94-0 Ῠ ù Kanamycin Mono Sulphate: 03G214: Kanamycin Monosulfate: 03SF033: Kanamycin monosulphate: 03SJ014 Ῠ ù Kanamycin sulfate: 02H007: 59-01-8 Ῠ ù Kanamycin Sulfate(Sterile) 04SC008: 8063-7-8 Ῠ ù Kanamycin sulphate

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  • Comparison of the SARS-CoV-2-inactivating activities

    2021-4-27 · SARS-CoV-2-infected VeroE6/TMPRSS2 cells were cultured in virus growth medium (VGM) containing Dulbecco's modified Eagle's medium (Nissui Pharmaceutical Co., Ltd, Tokyo, Japan) and 1% fetal bovine serum (FBS), 2 mM L-glutamine (Fujifilm Wako Pure Chemical Co., Ltd, Osaka, Japan), 100 μg/mL of kanamycin (Meiji Seika Pharma Co., Ltd, Tokyo ...

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  • Non-pathogenic Escherichia coli acquires virulence by ...

    2020-4-23 · ICR male mice (6 weeks of age) were given drinking water containing kanamycin monosulfate (0.6 mg/ml) for 24 h, and administered 0.2 ml of bacterial solution (10 7 CFU) by gavage. The mice were individually housed in clean cages. On days 1, 3, and 6 after the bacterial administration, their feces were collected for 2 h and weighed.

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  • Plant Growth and Agrobacterium-mediated Floral-dip ...

    Kanamycin VWR International, Radnor, PA 200004-444 Kanamycin monosulfate MES Bioworld, Dublin, OH 41320024-2 MES, Free Acid MS salt MP Biomedicals, Santa Anna, CA 092621822 Hoagland's modified basal salt mixture N6-benzylaminopurine (BA) ...

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  • Optimization of yeast-based production of medicinal ...

    2015-9-16 · Protoberberine alkaloids are bioactive molecules abundant in plant preparations for traditional medicines. Yeast engineered to express biosynthetic pathways for fermentative production of these compounds will further enable investigation of the medicinal properties of these molecules and development of alkaloid-based drugs with improved efficacy and safety.

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  • Morphological profiling of tubercle bacilli identifies ...

    2020-8-4 · Tuberculosis is a leading cause of death in the world and requires treatment with an arduous multidrug regimen. Many new tuberculosis drugs are in development, and the drug development pipeline would benefit from more rapid methods to learn drug mechanism of action and off-target effects. Here we describe a high-throughput imaging method for rapidly classifying drugs into categories based on ...

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  • A.T.Chem Supplier Asia Talent Chemical

    2014-10-29 · Kanamycin Mono Sulfate: 04SC008: 25389-94-0 Ῠ ù Kanamycin Mono Sulphate: 03G214: Kanamycin Monosulfate: 03SF033: Kanamycin monosulphate: 03SJ014 Ῠ ù Kanamycin sulfate: 02H007: 59-01-8 Ῠ ù Kanamycin Sulfate(Sterile) 04SC008: 8063-7-8 Ῠ ù Kanamycin sulphate

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  • Two Distinct Signaling Pathways in Hair Cycle Induction ...

    (Kyokuto Pharmaceutical, Tokyo) supplemented with 0.1 mM monoethanolamine/0.1 mM phosphoryl ethanolamine/0.5 ,M hydrocortisone/60 ,ug/ml kanamycin monosulfate at 37?C under an atmosphere of 5% C02/95% air. After being cultured in dishes precoated with collagen type I (Iwaki Glass, Tokyo…

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  • Application of Molecular Biology to Rice:

    2020-12-4 · The concentrations of kanamycin tested were from 0, 100, 250, 500, and 1000 mg/l. However, we used 300 mg/l of kanamycin for selection of transformed calli to make sure that we do not allow any non-transformed calli escaped from antibiotic selection. Publications. The above results have been written in a refereed article format in 2008.

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  • Cefotaxime | Duchefa | Bioz

    Remaining phages were counted every minute for 5 min postinfection. CON, control. (A) CPFX, ciprofloxacin; (B) CTX, cefotaxime; (C) KM, kanamycin; (D) MitC, mitomycin C. Article Snippet: Cefotaxime sodium salt, tetracycline hydrochloride, and kanamycin monosulfate were purchased from Duchefa Biochemie, Germany. Techniques: Adsorption, Incubation

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  • Disruption of pknG enhances production of gamma ...

    2014-4-1 · Bacterial strains and media. The bacterial strains and plasmids used in this study are listed in Table 1. E. coli strains were grown in Luria-Bertani medium (10 g L −1 tryptone, 5 g L −1 yeast extract, and 5 g L −1 sodium chloride) containing 50 μg mL −1 kanamycin at 37°C. C. glutamicum ATCC 13032 and all recombinant strains were grown in brain–heart infusion (BHI) medium (Becton ...

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  • Direct Agroinoculation of Maize Seedlings by Injection ...

    2021-2-27 · Abstract. Agrobacterium-based inoculation approaches are widely used for introducing viral vectors into plant tissues.This study details a protocol for the injection of maize seedlings near meristematic tissue with Agrobacterium carrying a viral vector.Recombinant foxtail mosaic virus (FoMV) clones engineered for gene silencing and gene expression were used to optimize this method, and its …

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  • Bacterial Expression of a Single-Chain Variable

    The cost of E. coli SHuffle ® expression system is much cheaper than its parental mAb production using mammalian hybridoma cell culture. When anti-GAA scFv was folded in cytoplasm compartment of E. coli SHuffle ®, in vitro time- and cost-consuming refolding steps could be avoided. In addition, protocol of anti-GAA scFv-based icELISA is simple ...

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  • Isolation and partial characterization of novel genes ...

    2009-6-5 · Cells harbouring the recombinant plasmids were first grown to OD 0·6 in Luria-Bertani (LB) broth containing 25 μg ml −1 of kanamycin and 34 μg ml −1 of chloramphenicol at 37°C and 200 rev min −1, and then expression of the C67-1 gene was induced by adding 0·5 mmol l −1 IPTG in the medium.

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  • Kanamycin sulphate | VWR

    Kanamycin sulphate 50 mg/ml in solution, Ultra Pure Grade. Catalog Number: (E713-20ML) Clearance. Supplier: VWR Chemicals. Description: Kanamycin is an aminoglycoside antibiotic that inhibits protein synthesis in gram-negative and gram-positive bacteria and in mycoplasma.

    Get Price
  • Kanamycin B | CAS#4696-76-8 | protein translation ...

    Kanamycin B is an inhibitor of protein translation and mammalian RNA splicing. It inhibits growth of gram negative bacteria, prevents formulation of the initiation complex, and decreases action potential amplitude in cardiac pacemaker cells.

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  • CAS No. 25389-94-0 | Chemsrc

    Isoarnebin 4, Shikonin S, Tokyo Violet. CAS Number: 517-89-5. Molecular Weight: 288.30. Empirical Formula (Hill Notation): C 16 H 16 O 5. Product Number Product Description SDS; PHL89791:

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  • Molecules | Free Full-Text | Determination of

    2021-5-20 · Within the mixture there is 1L of tryptic soy broth, .05g of kanamycin monosulfate, and 5g of the genetically altered E. coli. Once the E. coli have been left to grow for a 24hr period at 37°C, they are then placed within a bioreactor to promote futher growth and proinsulin production.

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  • 517-89-5 | Sigma-Aldrich

    A signal-on fluorescent aptasensor based on gold nanoparticles for kanamycin detection Yimeng Sun,a Tong Qi,a Yan Jin,ac Lijuan Liang *a and Jianlong Zhaoab This study describes the development, verification and practical application of an aptasensor for the

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  • step - insulinprocess

    2010-11-1 · A 3.0 g L −1 solution of kanamycin monosulfate (Agri-Bio, Miami, FL) was combined with 2 mL L −1 surfactant (Silwet L-77, Helena Chemical Co., Collierville, TN) and 500 mL deionized water in a 1 L spray bottle. A stream of approximately 1 mL was sprayed into the leaf whorl of a randomly selected stalk in each sugarcane stool. Sprayed stalks ...

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  • A signal-on fluorescent aptasensor based on gold ...

    1994-4-15 · Nonspecific esterase staining was performed using a commercial kit (Muto Pure Chemical, Tokyo, Japan). THP-1 and HL-60 cells, provided by Japanese Cancer Research Resources Bank (Tokyo, Japan), were cultured in RPMI 1640 supplemented with 15 mmol/L HEPES, 80 mg/L of kanamycin monosulfate, and 5% or 10% FBS, respectively.

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  • Transmission of a Sugarcane yellow leaf virus

    Phospholipase A2 (PLA2) is activated in spermatozoa in response to progesterone and Ca2 ionophores, but to our knowledge, no study has yet reported zona pellucida (ZP)-induced activation of PLA2. We investigated whether PLA2 is involved in ZP-stimulated acrosomal exocytosis, if Ca2 is required for activation of PLA2, and signal transduction pathways modulating PLA2 using guinea pig sperm as a ...

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  • Improvement of Hepatocyte Viability After

    2014-2-17 · kanamycin, 100 U/ml penicillin, 100 U/ml streptomycin, and 10% DMSO. Oligosaccharides and cyclic sugars were added to this medium at concentrations of 0.05, 0.1, or 0.2 mol/L. Dextran was added at 3%. One milli-liter of cell suspension containing 5 × 106 cells was quickly transferred to a 2.0-ml freezing tube and placed

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  • Kantrex (Kanamycin) - Drug Info, Side Effects,

    2008-6-22 · (Kanamycin Injection, USP) 1 g per 3 mL. Kanamycin sulfate is an aminoglycoside antibiotic produced by Streptomyces kanamyceticus. It is C 18 H 36 N 4 O 11 • 2H 2 SO 4.D-Streptamine, O-3-amino-3-deoxy-α-D-glucopyranosyl • (16)-O- [6-amino-6-deoxy-α-D-glucopyranosyl- (14)]-2-deoxy, sulfate 1:2 (salt). It consists of two amino sugars ...

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  • Measurement of the Promoter Activity in Escherichia coli ...

    2021-4-30 · Kanamycin Monosulfate (Nacalai. Tesque, catalog number: 19839-44) 17. Plasmid DNA Extraction Mini Kit (Favorgen, catalog number: FAPDE 001) 18. BigDye ® Terminator v3.1 Ready Reaction Mix (Applied Biosystems, catalog number:4337455) 19. IPTG (Nacalai. Tesque, catalog num. ber: 06289-67) 20. 3 M sodium acetate (Nacalai. Tesque, catalog number ...

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  • JCI - Enteroaggregative Escherichia coli expresses a

    2000-6-15 · Transformants were selected by growth on Luria broth (LB) agar with kanamycin (30 μg/mL), and the correct plasmid was identified by restriction pattern. This plasmid was then electroporated into the conjugative strain S17-1(λpir), and was conjugated into 042 by filter mating. Transconjugants, predicted to arise by chromosomal insertion of the ...

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  • Trends in Antimicrobial-Drug Resistance in Japan -

    2010-12-17 · Yoshichika Arakawa, Department of Bacterial and Blood Products, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan; fax: +81-42-561-7173 Return Address Send To

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  • English | World Gastroenterology Organisation

    Kanamycin (an injectable agent), moxifloxacin, prothionamide, clofazimine, isoniazid, pyrazinamide and ethambutol, given together in an initial phase of 4 months (with the option of extending to 6 months if the patient is still positive on a sputum smear at the end of month 4).

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  • VETB (Derwent Veterinary Drug Backfile)

    lo osaka and tokyo,jap. so antimicrobial agents chemother. (15, no.3, 392-95, 1979) la english . dt journal . fs r-x 1976-1982 . sh m microbiology . it antiseptic quinoxaline carbadox /taito pfizer olaquindox /nippon indene . pleuromutilin /sankyo protozoacide nitro imidazole metronidazole

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  • Set up new listing | Where to Buy Chemicals

    Set up new listing. Each single entry costs £38.00 Euro 50.00 USD 60.00 Ex vat. Orders for multiple entries will reduce the cost per entry and we will email accurate quotations once this form is submitted where you can cancel or amend any order following your quotation. Duration 1 year.

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  • Influence of Hydroxyl Groups on the Cell Viability of ...

    2016-9-1 · Polyhydroxyalkanoates (PHAs) are biopolyesters that have been studied as tissue engineering materials because of their biodegradability, biocompatibility, and low cytotoxicity. In this study, poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-2,3-dihydroxybutyrate) [PHBVDB] containing hydroxyl groups was produced by recombinant Ralstonia eutropha. R. eutropha were constructed to …

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