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application of yinzhijiedukeli for pseudorabies

  • PCR detection of pseudorabies virus and clinical application

    2017-3-9 · PCR detection of pseudorabies virus and clinical application Abstract:Pseudorabies virus (PRV) in GenBank. The complete sequence of gE gene, using Primer5.0 software in the conserved region of the gene, a pair of primers, amplified fragments of 1742bp.

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  • Study on the application of pseudorabies control and ...

    In order to effectively differentiate between gene-deleted vaccine and wild-type isolates of Pseudorabies virus(PrV), a PCR method, based on sequences of gE and gI of the PrV genome, was established after selection of the optimal reaction conditions. By applying ...

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  • US Patent Application for Porcine Pseudorabies Virus

    A plan for pseudorabies control was evaluated in 3 breeding pig farms and the experimental pigs were immunized with a pseudorabies gE deletion vaccine. The PRV-gE antibody was tested every 3 months, and the positive pigs were eliminated and newly introduced breeding pigs were quarantined and bio-safety measures were taken regularly, e.g., deratization and disinfection.

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  • Construction of recombinant pseudorabies viruses by

    The present invention relates to the technical field of porcine pseudorabies virus, and more particularly to a porcine pseudorabies virus (PRV)-YF strain, with the preservation No. of CCTCC No. V201502. The present invention also provides an application of the porcine pseudorabies virus (PRV)-YF strain in preparing an inactivated vaccine of the porcine pseudorabies virus, the inactivated ...

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  • Development of pseudorabies virus strains expressing

    Construction of recombinant pseudorabies viruses by using PRV BACs deficient in IE180 or pac sequences: Application of vBAC90D recombinant virus to production of PRV amplicons. Author links open overlay panel L. Lerma A.L. Muñoz S. Wagner M. Dinu B. Martín E. Tabarés.

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  • Establishment and Application of Transcription

    The development of a strain isogenic to PRV152 and differing only in the fluorescent reporter would have great utility for dual transsynaptic tracing. In this report, we describe the construction, characterization, and application of strain PRV614, a PRV-Bartha derivative expressing a …

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  • Application of in situ viral nucleic acid hybridization to ...

    2020-10-13 · Application of in situ viral nucleic acid hybridization to the study of Aujeszky's disease (pseudorabies) in calves Harold Antonio McAllister Iowa State University Follow this and additional works at:https://lib.dr.iastate.edu/rtd Part of theAnimal Sciences Commons, and …

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  • Pseudorabies (Aujeszky's disease) virus DNA detection in ...

    2020-10-16 · Pseudorabies (Aujeszky's disease) virus DNA detection in swine nasal swab and oral fluid specimens using a gB-based real-time quantitative PCR . ... tematic application of highly effective DIVA vaccines and ELISAs proved highly effective in the control and/or elimination of PRV.

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  • Application of a quantitative algorithm to restriction ...

    Fingerprint Dive into the research topics of 'Application of a quantitative algorithm to restriction endonuclease analysis of Aujeszky's disease (pseudorabies) virus from a geographically localized outbreak'. Together they form a unique fingerprint. Sort by Weight Alphabetically

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  • Anti-Pseudorabies Virus antibody (ab3534) | Abcam

    Pseudorabies virus (PRV) is a member of the neurotropic viruses known as alphaherpesviruses. PRV is primarily a disease of swine which serve as a reservoir for the virus and the principal source of natural infection for a diverse range of secondary hosts, including …

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  • Development of a polymerase chain reaction assay for

    Aujeszky's disease, also known as pseudorabies causes severe economic losses in swine industry and affects the pig husbandry all over the world. The conventional diagnostic procedure is time-consuming and false-negative results may occur in submissions from latently infected animals. The development, optimization and evaluation of a polymerase ...

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  • Dot-enzyme immunoassay for visual detection of

    2021-2-2 · A modified solid-phase enzyme immunoassay (EIA) is described for the visual detection of anti-pseudorabies virus (anti-PRV) antibody in porcine serum. Dots of PRV antigens were adsorbed to nitrocellulose paper (hence the name dot-EIA), and the remaining nonspecifically reactive sites were blocked with bovine serum albumin or skim milk powder. After immersion in test serum, bound …

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  • Pseudorabies: adaptation of the countercurrent ...

    Pseudorabies: Adaptation of the Countercurrent Immunoelectrophoresis for the Detection of Antibodies in Porcine Serum G. Papp-Vid and G. C. Dulac* ABSTRACT INTRODUCTION A countercurrent immunoelectrophoresis test was developed for the detection of precipitating antibodies to pseudorabies virus in pig serum.

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  • CN103739697A - Recombinant protein for resisting

    The invention discloses a recombinant protein for resisting porcine pseudorabies virus reproduction, and application of the recombinant protection to synthesis of a medicine for resisting porcine pseudorabies virus reproduction. The recombinant protein for resisting porcine pseudorabies virus reproduction is a protein with an amino acid sequence consisting of an amino acid sequence shown as ...

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  • Application Progress of Procine Pseudorabies Virus gE ...

    Abstract: With mass immunization application of pseudorabies virus (PRV) gE gene deleted attenuated vaccine in China, it played an important role in prevention and control of porcine pseudorabies, meanwhile it also needed to establish a differential diagnosis technology for gE gene deleted vaccine matching to eliminate virus affected swine and reduce immune pigs to be killed, and …

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  • Expression of Truncated gE Gene of Pseudorabies

    With the application of gE gene deleted pseudorabies virus(PRV) vaccine worldwide, a corresponding differential diagnosis based on gE glycoprotein is needed in the project of PRV eradication. In this study, PRV gE gene without signal and transmembrane region was amplified by PCR and cloned into pGEX 6P 1, generated pGEX gE. After transformation of BL21 with pGEX gE, an expressed fusion protein ...

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  • USDA APHIS | PRV Program Standards

    2003-11-1 · 2. All swine must originate from a State that has achieved Pseudorabies Eradication Program status of Stage III, IV, or V; or 3. All swine must originate in a pseudorabies-monitored feeder-pig herd; or 4. All swine are found negative to an official pseudorabies test conducted 30 days or less prior to presentation at the market.

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  • Development and preliminary application of gE

    Development and preliminary application of gE enzyme-linked immunosorbent assay for detection of the antibody to gE protein of Pseudorabies virus in pigs - Volume 2 Issue 2

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  • Antiviral activity of Piscidin 1 against pseudorabies ...

    2019-7-31 · RESEARCH Open Access Antiviral activity of Piscidin 1 against pseudorabies virus both in vitro and in vivo Han Hu1,2, Nan Guo1, Shuhua Chen3, Xiaozhen Guo1, Xiaoli Liu1, Shiyi Ye3, Qingqing Chai4, Yang Wang2, Binlei Liu2 and Qigai He1,3* Abstract Background: Swine-origin virus infection spreading widely could cause significant economic loss to porcine industry.

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  • DNA sequence of the gene for pseudorabies virus

    The DNA sequence was determined for a region of the pseudorabies virus (PRV) genome to which a mutation defining resistance to a monoclonal antibody has been mapped (M. W. Wathen and L. M. K. Wathen, J. Virol., 51:57-62, 1984). This sequence was found to contain an open reading frame that did not include an amino acid sequence directing N ...

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  • Construction and Transposon Mutagenesis

    2016-8-16 · 近期,来自吉林大学动物科学学院的研究人员在《Virus Research》发表题为“Pseudorabies virus can escape from CRISPR-Cas9-mediated inhibition”的研究成果。这项研究发现,伪狂犬病病毒(Pseudorabies virus)可以逃避CRISPR-Cas9介导的抑制

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  • Fluorescence Signal Amplification of Quantum Dots

    A full-length clone of the 142-kb pseudorabies virus (PRV) genome was constructed as a stable F plasmid in Escherichia coli. The clone, pBecker1, was colinear with PRV-Becker genomic DNA, lacking detectable rearrangements, deletions, or inversions. The transfection of pBecker1 into susceptible eukaryotic cells resulted in productive viral infection. Virus isolated following transfection was ...

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